Journal: Nature Communications
Article Title: Transgenerational inheritance of diminished ovarian reserve triggered by prenatal propylparaben exposure in mice
doi: 10.1038/s41467-025-63440-z
Figure Lengend Snippet: a The methylation levels of CpG site −1555 to TSS in the promoter region of Rhobtb1 following PrP exposure ( n = 5 biological replicates). Data are presented as mean ± SEM (one-way ANOVA). b , c Protein levels of RhoBTB1 in KGN cells following PrP exposure. Data from ( c ) represent three independent experiments (mean ± SEM; one-way ANOVA). d AMH levels in KGN cells following PrP exposure ( n = 3 biological replicates). Data are presented as mean ± SEM (one-way ANOVA). e E2 levels in KGN cells following PrP exposure ( n = 3 biological replicates). Data are presented as mean ± SEM (one-way ANOVA). f , g Violin plot showing the apoptosis ratio of KGN cells after PrP exposure ( n = 3 biological replicates; one-way ANOVA). The black dotted line represents median ratio. h , i The protein levels of BAX, BCL-2, and active-Caspase3 in KGN cells tested by western blotting following PrP exposure. Data from ( i ) represent three independent experiments (mean ± SEM; one-way ANOVA). j Representative images showing the infection of adenovirus with and without encoding Rhobtb1 in KGN cells. The experiment was repeated three times. k , l Protein levels of RhoBTB1 in GCs detected by western blotting following RhoBTB1 overexpression. Data from ( l ) represent three independent experiments (mean ± SEM; unpaired two-tailed t -test). m AMH levels in GCs following RhoBTB1 overexpression ( n = 5 biological replicates). Data are presented as mean ± SEM (unpaired two-tailed t -test). n E2 levels in GCs following RhoBTB1 overexpression ( n = 5 biological replicates). Data are presented as mean ± SEM (unpaired two-tailed t -test). o , p Violin plot showing the apoptosis ratio of GCs after RhoBTB1 overexpression ( n = 3 biological replicates; unpaired two-tailed t -test). The black dotted line represents median ratio. q , r The protein levels of BAX, BCL-2, and active-Caspase3 in GCs detected by western blotting following RhoBTB1 overexpression. Data from ( r ) represent three independent experiments (mean ± SEM; unpaired two-tailed t -test). Source data are provided as a Source Data file.
Article Snippet: The Antibodies were purchased from different companies: Cell Signaling Technology for ERK (Extracellular Signal-Regulated Kinase; 4695T), p-ERK (Phosphorylated ERK; 4370T), p38 (p38 Mitogen-Activated Protein Kinase; 8690T), p-p38 (Phosphorylated p38; 4511T), JNK (c-Jun N-terminal Kinase; 9252T), and p-JNK (Phosphorylated JNK; 4668T); Abclonal for β-ACTIN (AC038), active-Caspase3 (A19654), BCL-2 (A19693), BAX (A12009), SOD2 (A19576), BMP15 (A7321), CAT (A11777), HSD17B7 (A17157), and CTSK (A1782); Proteintech for FGF18 (60341-1-lg); Immunoway for RhoBTB1 (YT4084); Servicebio for HRP conjugated goat anti-rabbit IgG (GB23303), and Alexa Fluor® 488-conjugated goat anti-rabbit IgG (GB25303).
Techniques: Methylation, Western Blot, Infection, Over Expression, Two Tailed Test
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